With the rapid rise of periodical on the Web, the study on the quality evaluation standards for Websites has been given more and more attention for its future development. Based on the quality evaluation process currently involved in the Websites and taking four related aspects into consideration , such as the whole systems, Web content, Web design and Web submission, this paper puts forward a multilayer level indicator system for quality evaluation and sets out a detailed index. At the same time, this paper discusses the operation of quality evaluation for periodical Websites based on the analytical hierarchy process.